Adenosine 3'5'-monophosphate phosphodiesterase of buffalo spermatozoa

Structure of sporostatin (M5032), an inhibitor of cyclic adenosine 3',5'-monophosphate phosphodiesterase.

EnzymeAssay Bovine heart CAMP-PDEactivity was measured as follows: The reaction mixture contained in a final volume of 1.0 ml, Tris-HCl buffer (pH7.0) 40him, MgSO4 2mM, CAMP0.6mMas a substrate and the enzyme 7.5mU/ml. The reaction was started by adding the substrate. Incubation was carried out 30°C for 30 minutes, and the reaction was terminated by adding 0.1ml of 55% perchloric acid, and the m...

Properties of adenosine 3',5'-monophosphate-dependent protein kinases isolated from bovine epididymal spermatozoa.

Three protein kinases were separated from bovine epididymal spermatozoa by using DEAE-Sephadex ion exchange chromatography and Sephadex G-100 gel filtration. The molecular weights of these three kinases were estimated to be 120,000, 78,000 and 56,000 by the use of Sephadex G-100 gel filtration. The activity of all three kinases was greatly enhanced by cyclic adenosine 3’,5’-monophosphate (cycli...

Hydrolytic rupture of ascorbate by adenosine 3':5'-cyclic monophosphate phosphodiesterase.

An effect of insulin on adipose-tissue adenosine 3':5'-cyclic monophosphate phosphodiesterase.

1. 3':5'-Cyclic nucleotide phosphodiesterase activity was measured in homogenates prepared from epididymal fat-pads and isolated fat-cells incubated in the absence and presence of insulin. 2. Homogenates of insulin-treated tissues showed an increase in phosphodiesterase activity compared with controls. No effect of insulin was observed when the hormone was added directly to homogenates. 3. Ther...

Inhibitors of adenosine 3',5'-cyclic monophosphate phosphodiesterase in Daphne genkwa Sieb. et Zucc.

Adenosine 3',5'-cyclic monophosphate (cAMP) phosphodiesterase inhibition testing provides a useful means for the screening of biologically active compounds contained in medicinal plants. We have identified cAMP phosphodiesterase inhibitors contained in various medicinal plants.2a-k) The present paper deals with the identification of cAMP phosphodiesterase inhibitors contained in the flowers and...